Risk of bias assesment, Quadas-2 criteria.

Population
1.a consecutive or random sample of patients were enrolledyesnounclear.
2.enrolment preceded verification of the disease status (case-control design was avoided) yesnounclear.
3.inappropriate exclusions were avoided yesnounclear.
4.all included patients were included in the data analysis yesnounclear.
Index test(s)
5.the index test was done in all patients yesnounclear.
6.the index test was interpreted without knowledge of the disease statusyesnounclear.
7.a threshold was used to interpret the index test yesnounclear.
8.the threshold was pre-specified yesnounclear.
Reference standard
9.the disease status was verified in all patients yesnounclear.
10.the disease status was verified without knowledge of the index test result yesnounclear.
11.the same reference standard was used in all patientsyesnounclear.
12.the reference standard allowed correct classification of the disease statusyesnounclear.