RNA-seq of Xylella fastidiosa OMVs and WCL

Xylella fastidiosa is a Gram-negative plant pathogen responsible for severe diseases in a variety of economically important crops. A critical aspect of its pathogenicity is the production of outer membrane vesicles (OMVs). While some knowledge exists on the molecular cargo of X. fastidiosa (Xf)-OMVs, nucleic acid cargoes remain unknown. Other bacterial species show modulation of host immune signaling by OMV-packaged RNAs, aiding bacterial infection success. In order to understand if Xf employs a similar mechanism, we sequenced total RNA extracted from OMVs and compared it to RNA extracted from whole bacterial cells.