Figure 1efg

Auxin repressors expressed in maize inflorescence exhibited variable auxin-induced degradation dynamics. E and G, ZmIAAs degrade at different rates that are dependent upon both repressor (E) and receptor (G) identities. Yeast strains expressing YFP-tagged ZmIAAs and either Arabidopsis TIR1 or AFB2 auxin receptor were exposed to 1 ?m auxin or mock treatment (95% [v/v] ethanol) at 0 min, and fluorescence measurements were acquired on a flow cytometer. Data from two replicates are shown. F, YFP:ZmIAA degradation half-lives were calculated from cytometry data in E and G and are presented with 95% confidence intervals.
Conclusion:
E and G, ZmIAAs degrade at different rates that are dependent upon both repressor (E) and receptor (G) identities. Yeast strains expressing YFP-tagged ZmIAAs and either Arabidopsis TIR1 or AFB2 auxin receptor were exposed to 1 μm auxin or mock treatment (95% [v/v] ethanol) at 0 min, and fluorescence measurements were acquired on a flow cytometer. Data from two replicates are shown. F, YFP:ZmIAA degradation half-lives were calculated from cytometry data in E and G and are presented with 95% confidence intervals. Yeast autofluorescence was cancelled out using non-fluorescent yeast. All yeast strains were tested for basal fluorescence prior to treatment from over 50 minutes of growth.