Gene expression profile at single cell level of splenocytes from wild-type and S100a9 knock-out mice repeated social defeat stress

Psychological trauma increases inflammation. In a mouse model of psychological trauma (repeated social defeat stress), we used single cell RNA sequencing to assess the gene expression in splenocytes ± psychological trauma as well as without S100a9 (gene of interest) 12 Mice (3 WT control, 3 KO control, 3 WT stress, 3 KO stress) underwent a standard 10 day repeated social defeat stress paradigm followed by 1 day of behavior testing. The following day (day 12), splenocytes were harvested and utilized for single cell RNA sequencing.心理创伤可加剧炎症反应。本研究在心理创伤（重复性社交挫败应激）小鼠模型中，采用单细胞RNA测序（single cell RNA sequencing），对伴/不伴心理创伤以及缺失目的基因S100a9的脾细胞基因表达水平进行评估。实验共使用12只小鼠，分为4组：野生型（Wild Type, WT）对照组（3只）、敲除型（Knock Out, KO）对照组（3只）、野生型应激组（3只）、敲除型应激组（3只）。所有小鼠均接受标准的10天重复性社交挫败应激造模范式，随后开展1天的行为学测试。造模及行为测试结束后的次日（第12天），采集脾细胞并用于单细胞RNA测序实验。