RNA Seq analysis of NKX2-5 Null and Het human embryonic stem cells in cardiomyogenesis

Differentiation of human pluripotent stem cells (hPSCs) can be used to model human heart development and, in turn, to analyze the developmental consequences of genetic abnormalities. Here, we deleted NKX2-5, a critical component of the cardiac gene regulatory network, in human embryonic stem cells (hESCs) and identified a novel genetic interaction between NKX2-5 and HEY2 that is required for heart development Overall design: Comparision of Null and Het NKX2-5 hESCs