Truncation of Pik3r1 Causes Severe Insulin Resistance Uncoupled From Obesity and Dyslipidaemia by Increased Energy Expenditure

Both PIK3R1(Y657X) mutant and wild-type C57Bl/6J mice were maintained on chow until 16 weeks old, at which time they were fasted for 16 hours and then refed chow for 6 hours. The tissues were harvested and snap-frozen at three time points: chow diet before overnight fast (ad libitum chow, ALC), after 16 hour fast (overnight fast, ONF), and after 6 hour refeeding (refed 6 hours, RF6). RNAseq profiles of PIK3R1(Y657X) mutant and wild-type C57Bl/6J mice in three states: fasting, refed, and chow.携带PIK3R1(Y657X)突变的小鼠与野生型C57Bl/6J小鼠均以常规饲料（chow）饲喂至16周龄，随后进行16小时禁食，之后恢复饲喂常规饲料6小时。分别在三个时间点采集组织并快速冷冻：过夜禁食前的常规饲料自由采食状态（ad libitum chow, ALC）、16小时过夜禁食后（overnight fast, ONF）以及复饲6小时后（refed 6 hours, RF6）。本数据集涵盖了PIK3R1(Y657X)突变型与野生型C57Bl/6J小鼠在禁食、复饲及常规饲喂三种状态下的RNAseq图谱。