Expression data of Oral squamous cell carcinoma (OSCC) cell lines CAL27, SCC4 and SCC25 treated with oral commensals

Oral bacterial species Streptococcus mitis, Neisseria flavescens, and Haemophilus parainfluenzae, and Porphyromonas gingivalis were each co-cultured with the OSCC cell lines CAL27, SCC4 and SCC25 individually. Total RNA was extracted from the cell lines, followed by gene expression profiling with microarray analysis. OSCC cell lines were allowed to attach for 24 h before the oral bacteria were added. After 24 h actively growing bacteria in their mid-log stage were added to OSCC cells, resulting in a total of 12 co-cultures (3 cell lines* 4 bacterial species). All the co-cultures were performed in triplicates