MR1 presents sulfated bile acid as a self antigen for MAIT cells [PBMC]

To investigate the effect of cholic acid sulfate to MAIT cells, PBMCs were stimulated with each antigen (5-OP-RU, cholic acid sulfates; CA3S and CA7S) and scRNA sequencing was performed. the homeostatic related genes expressed in cholic acid sulfate stimulation, while the inflammatory related gene expressed in 5-OP-RU stimulation. This analysis showed that cholic acid sulfate contributes to maintenance of MAIT cells. PBMCs were stimulated with 5-OP-RU, CA3S and CA7S. 17 days later, CD161+MR1-5-OP-RU tetramer+ cells were sorted and analyed using single-cell TCR- and RNA-seq. Hashtag sample definitions: Hash_tag_1, non-stimulated Hash_tag_2, 5-OP-RU (5-(2-oxo-propylidenamino)6-D-ribitylaminouracil) 10 M Hash_tag_3, Cholic acid 3-sulfate 1000 M Hash_tag_4, Cholic acid 3-sulfate 300 M Hash_tag_5, Cholic acid 7-sulfate 1000 M Hash_tag_6, Cholic acid 7-sulfate 300 M Hash_tag_7, Cholic acid 7-sulfate 100 M