Phyca

The project is being completed using a combination of Sanger di-deoxy terminator sequencing of random clones and a newer pyrosequencing technology (dubbed “454 sequencing”) that bypasses bacterial cloning and generates shorter sequence reads. The isolate used for the sequencing, LT1534, was produced by crossing field isolates from Tennessee (LT263) and Michigan (OP97) to produce an F1 population and then conducting two consecutive rounds of backcrossing to LT263 to achieve a moderate level of inbreeding.本项目采用随机克隆桑格双脱氧终止法测序（Sanger di-deoxy terminator sequencing）与新型焦磷酸测序技术（pyrosequencing，俗称"454 sequencing"）相结合的方案完成测序工作，该新型技术可绕过细菌克隆步骤，生成更短的序列读长。本次测序所用的分离株LT1534，通过将来自田纳西州的田间分离株LT263与密歇根州的OP97杂交得到F1群体，随后连续进行两轮回交至LT263，以实现中等程度的近交。

搜集汇总

背景与挑战

背景概述

该数据集基于菌株LT1534的测序项目，结合了Sanger测序和454测序技术。菌株LT1534通过田纳西州和密歇根州野外分离株杂交并回交培育而成。