Transcriptomic profile of circulating memory CD4 T cells in active versus latent tuberculosis

Tuberculosis (TB) is responsible for the majority of mortality and morbidity associated with infectious diseases worldwide. The characterization of exact molecular components of immune response associated with protection against TB may help design more effective therapeutic interventions. In this study, we aimed to characterize the immune signature of memory T cells associated with active versus latent infection with Mycobacterium tuberculosis. Transcriptomic profiling using RNA sequencing was performed on memory CD4 T cells isolated from individuals with active TB (at diagnosis and 2 months post treatment), latent TB, as well as from TB negative healthy controls. Overall, we found specific gene signatures for each cohort that could successfully discriminate between individuals with active TB at diagnosis, treated active TB, latent TB and healthy controls. RNA-sequencing of sorted memory CD4 T cells from cryopreserved PBMC of 25 subjects with active tuberculosis a diagnosis (including 21 with an additional sample collected at 2 months post treatment), 40 subjects with latent tuberculosis infection and 20 tuberculosis negative healthy controls Note: The submitter declares that the raw data cannot be made available via any mechanism due to patient privacy concerns