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In vitro Radioligand Competition Binding Assay.

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Figshare2015-12-02 更新2026-04-29 收录
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https://figshare.com/articles/dataset/_In_vitro_Radioligand_Competition_Binding_Assay_/298736
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Ligand binding affinity (pIC50) were measured on intact COS-7 cells transfected with wild type and mutant receptors by competition binding using 125I-His5-D-Tyr6-GnRH1.Bmax = % of total radioligand bound were expressed relative to wild type receptor without NBI-42902.nbNo measurable binding.Values are mean ± SEM of three or more independent experiments, converted to nM (in parentheses).Significant difference of NBI-42902 treatment on Bmax is represented by an asterisk * (p
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2015-12-02
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