High depth sequencing of BrU-labeled nascent RNA in METTL3 depleted cells.

BrU incorporation into nascent RNA followed by immunoprecipitation with a BrU specific antibody. The nascent RNA was subsequently eluted with BrU competition. HEK293T cells, treated with METTL3 siRNA, were incubated with 2 mM bromouridine for 15 min labeling time and chased with 20 mM uridine for 60 min. Nascent RNA was purified during BrU-IP with anti-BrdU.