Human Skin Microbiome Data (16S rRNA sequencing)

16S rRNA sequencing data on human skin microbiome samples collected before and after swimming in the ocean. This dataset contains raw sequencing data contained in fasta and qual files produced from an Ion Torrent PGM sequencer. There were 2 sampling occurrences (041218 and 092718) and each occurrence has an associated fasta and qual file. This dataset contains the 041218 sampling data only due to storage restrictions. The other dataset is published separately.
Our research has shown that the human skin microbiome is altered after swimming in the ocean. Normal commensals were washed off and simultaneously, exogenous bacteria were deposited on the skin. QIIME was used for initial analysis and indicated that the abundance and diversity of microbial communities on the skin increased after swimming and these changes persisted for more than 24 hours. Downstream analysis using PICRUSt to predict functional metagenomics indicated that there was an increase in antibiotic resistance genes, antibiotic biosynthesis genes, and virulence factor genes on the skin after ocean water exposure.

本研究收集了人类皮肤微生物群样本在海洋游泳前后16S rRNA测序数据。本数据集包含由Ion Torrent PGM测序仪生成的fasta和qual文件中的原始测序数据。该数据集涵盖了两次采样事件（041218和092718），每次采样事件均对应一个fasta和qual文件。由于存储限制，本数据集仅包含041218的采样数据，而另一数据集已单独发布。研究表明，海洋游泳后人类皮肤微生物群发生改变，正常共生菌被冲走，同时，外源性细菌沉积于皮肤上。初始分析采用QIIME工具，结果显示游泳后皮肤上微生物群的丰富度和多样性增加，且这些变化持续超过24小时。后续分析利用PICRUSt工具预测功能宏基因组学，表明皮肤暴露于海水后，抗生素耐药基因、抗生素生物合成基因和致病因子基因的数量有所增加。