DNA methylation of miRNA genes in HMEC and HMF. Homo sapiens

Normal cell type specific DNA methylation of miRNA genes. HMEC and HMF represent two distinct differentiated cell type present in mammary gland each with a distinct phenotype, a distinct epigenotype as well as distinct miRNA expression pattern. The aim of the study was to determine how epigenetic modifications including DNA methylation effect miRNA expression. Overall design: Two cell types HMEC vs. HMF. Biological replicates: 3 pairs of HMEC-HMF of 3 distinct genotypes. Immunoprecipitation using anti-Methylcytosine (5MeC) antibody.