scRNA sequencing of echovirus infected (and uninfected) neonatal mouse livers across four IFN KO mouse genotypes.

The goal of this study was to understand how the liver responds to echovirus infection in a neonatal population. To do this, we used hFcRnTg32 mice, since these mice express the pan-echovirus receptor under its native promotor, and generated 3 additional IFN knockout genotypes on this background (hFcRnTg32: Ifnar-/-, Ifnlr-/-, Ifnar-/-Ifnlr-/-). Neonatal (7do) mice were then challenged with echovirus to observe how liver resident cell types were affected in the context of different arms of the innate immune system at a single cell resolution.