TNF-alpha bioassay

TNF-α production by PBMCs from 3 donors cultured on films synthesised from 3 batches of each optimal treatment type (180°C for 1.5h or NaOH for 2h) versus native chitosan for 24 hours. Symbols are grouped based on the batch of chitosan films made. PBMCs were cultured alone with or without 100 ng/ml LPS as a positive or negative control, respectively. TNF-α values were analysed for statistical significance using a general linear model separating batch, donor and treatment effects. There were significant differences between treatment groups and multiple comparisons were performed to compare groups using Tukey's correction for multiple pairwise tests. N=9.