Effect of IR coexpression on the pharmacological properties of β2AR in HEK-293 cells.

ND – not detected.HEK-293 cells expressing HA-β2AR alone or HA-β2AR together with IR at a 1∶1 cDNA ratio were incubated with [125I]-iodopindolol and increasing concentrations of the indicated ligands (10–12 to 10–5 M final concentration). When the concomitant effect of pindolol and insulin was tested, insulin was added to a 0.1 µM final concentration. IC50 values were generated using a sigmoidal dose-response curve fit (GraphPad Prism 5.0). Data are expressed as the means ± S.E. of three independent experiments performed in triplicate.Effect of IR coexpression on the pharmacological properties of β2AR in HEK-293 cells.