snATAC-seq of developing marmoset female and male gonads across development

This study used droplet-based snATAC-seq to profile the chromatin accessibility landscape of 22,032 nuclei in the developing marmoset female and male gonads across several timepoints during development, from gestational day (GD) 74 until 3 weeks after birth (wo). Embryos were collected via hysterotomy. A specialized veterinarian performed all surgical procedures, including anesthesia and analgesia. Additionally, postnatal samples from newborn and 3 weeks-old marmoset were collected following euthanasia. Single-cell barcoding and library preparation were performed using Chromium Single Cell 3' Reagent Kits (v3 chemistry) and the Chromium Controller instrument (10x Genomics). Sequencing of the libraries was carried out on the Illumina NextSeq 500/550 and initial data processing was performed using Cellranger.