Heterotrimeric G-protein interactions are conserved despite regulatory element loss in some plants

Purpose: To study the shared transcriptional network between suppression transgenic plants of BdG and overexpresssion plants of SiRGS in B. distachyon. Method: Total RNA was isolated from 2 independent transgenic lines of amiR-BdG and Bd-SiRGS-OE. RNA-seq library preparation, sequencing and initial quality control analysis were performed by Novogene Inc. The sequences were analyzed for quality using FastQC. Transcripts per million (TPM) abundance was estimated by pseudo-aligning the reads with that of the k-mer index available from the reference genome of B. distachyon from Phytozome using Kallisto. Differentially expressed genes were sorted using Sleuth program with likelihod ration cutoff of false discovery rate (FDR) < 0.05. The log2 fold change was calculated from the TPM values derived for the wild-type and transgenic plants. Result: The transcriptome data showed more that 60% of overlap between amiR-BdG and Bd-SiRGS-OE. Among common genes more than 90% showed similar expression pattern when screened for several developmental and hormonal signaling pathways. Overall design: B. distachyon was used as wild-type control. 2 independent transgenic lines of amiR-BdG and Bd-SiRGS-OE were used. The tissue were collected as whole seedlings at 2-week-old stage.