Fins as a surrogate for age-related changes of telomeres and DNA methylation in gonads

This is code and datasets accompanying "<b>Fins as a reliable surrogate tissue for age-related changes of telomeres and DNA methylation in gonads of a short-lived fish</b>" by Vrtílek et al. We collected fin and gonadal tissues from both females and males of the turquoise killifish (<i>Nothobranchius furzeri</i>) at three different ages, and extracted DNA to measure telomere length via terminal restriction fragment (TRF) analysis and global DNA methylation levels using double-digest restriction-associated DNA sequencing (ddRADseq). Our results show a notable correspondence between telomere length and DNA methylation patterns in fin and gonadal tissues. These findings support the use of fin biopsies as a non-lethal method for assessing ageing biomarkers in the gonads of small freshwater fish.The data comprise of telomere lengths measured from individual samples and read numbers per locus from libraries obtained from methylation-sensitive or non-sensitive sequencing (for more details on our methods, please, see the article).