Data from: Obtaining mtDNA genomes from next-generation transcriptome sequencing: a case study on the basal Passerida (Aves: Passeriformes) phylogeny.

Classically, the mitochondrial genome is sequenced by a series of amplicons using conserved PCR primers. Here we show how shot-gun transcriptome sequencing can be used to obtain the complete set of protein-coding genes from the mtDNA of four passerine bird species. With these sequences, we address the still unresolved basal Passerida relationships (Aves: Passeriformes). Our analysis suggests a new hypothesis for the basal relationships of Passerida, namely a clade grouping Sylvioidea and Passeroidea, with Paridae and Muscicapidae as successive sister groups to this clade. This study demonstrates the usefulness of next-generation sequencing transcriptome sequencing for obtaining new mtDNA genomes.传统上，线粒体基因组（mitochondrial genome）的测序多采用保守PCR引物介导的系列扩增子策略完成。本研究展示了如何通过鸟枪法转录组测序（shot-gun transcriptome sequencing），从四种雀形目鸟类（passerine bird）的线粒体DNA（mitochondrial DNA, mtDNA）中获取全套蛋白质编码基因。借助这些序列，我们探究了迄今仍未解决的雀下目（Passerida）基干系统发育关系（鸟纲: 雀形目）。我们的分析提出了雀下目基干系统发育关系的全新假说：莺总科（Sylvioidea）与雀总科（Passeroidea）构成单系支系，而山雀科（Paridae）和鹟科（Muscicapidae）依次为该支系的姊妹群。本研究证实了基于下一代测序的转录组测序技术在获取全新线粒体DNA基因组方面的应用价值。