SEQC Toxicogenomics Study: RNA-Seq data set

The comparative advantages of RNA-Seq and microarrays in transcriptome profiling were evaluated in the context of a comprehensive study design. Gene expression data from Illumina RNA-Seq and Affymetrix microarrays were obtained from livers of rats exposed to 27 agents that comprised of seven modes of action (MOAs); they were split into training and test sets and verified with real time PCR.本研究采用综合实验设计，评估了RNA测序（RNA-Seq）与微阵列（microarrays）在转录组谱分析中的比较优势。研究从暴露于27种受试物的大鼠肝脏组织中，获取了Illumina RNA测序（RNA-Seq）与Affymetrix微阵列的基因表达数据，上述受试物涵盖7种作用模式（Mode of Action, MOA）；随后将数据划分为训练集与测试集，并通过实时定量PCR（real-time PCR）完成验证。