Phylogenetic tree of the sdiA gene

The phylogenetic tree of Salmonella sdiA gene sequences demonstrate notable genetic diversity across various serovars. A major cluster includes numerous S. Enteritidis strains, such as "Slowbro," "Swellow," "Wailord," and others, which group closely together yet display clear internal branching—suggesting intra-serovar variation within S. Enteritidis. Additional S. Enteritidis strains like "Charizard," "Marill," and "Glaceon" form a distinct subcluster, reinforcing the notion of genetic divergence within the same serovar. Other serovars, including S. Typhimurium (strains 14028 and S34), S. Typhi (Salty24446 1972 and 2017), and S. Dublin (SAL-19-0015), form separate, well-supported clades, indicating serovar-specific evolutionary divergence.