Real-time microRNA quantitative PCR array of mouse bone marrow-derived osteoclasts II. Real-time microRNA quantitative PCR array of mouse bone marrow-derived osteoclasts II
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https://www.ncbi.nlm.nih.gov/bioproject/PRJNA703478
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资源简介：
Mouse bone marrow-derived OSCAR+CTR+ osteoclasts were harvested from osteoclast-miRNA knockdown (OCmiR-KD) mice or osteoclast-miRNA intact (OCmiR-Intact) mice at 2w after anterior cruciate ligament transection (ACLT) by magnetic-activated cell sorting. The cells were lysated by Trizol reagent for total RNA extraction. The miRNA were profiled using the QuantiMir RT Kit (System Biosciences) Overall design: miRNA qPCR expression profiling. Equal amount total RNA from osteoclasts of three independent OCmiR-KD mice and three OCmiR-Intact mice were pooled for gene expression analysis.
创建时间：
2021-02-21
