Short-chain cello-oligosaccharides: Intensification and scale-up of their enzymatic production and selective growth promotion among probiotic bacteria

We provide here the underlying data of the scientific publication "Short-chain cello-oligosaccharides: Intensification and scale-up of their enzymatic production and selective growth promotion among probiotic bacteria". Please find the abstract below: Short-chain cello-oligosaccharides (COS; degree of polymerization, DP≤6) are promising water-soluble dietary fibers. An efficient approach to their bottom-up synthesis is from sucrose and glucose using glycoside phosphorylases. Here, we show intensification and scale up (20 mL; gram scale) of COS production to 93 g/L product and in 82 mol % yield from sucrose (0.5 M). The COS were comprised of DP 3 (33 wt %), DP 4 (34 wt %), DP 5 (24 wt %) and DP 6 (9 wt %) and involved minimum loss (≤10 mol %) to insoluble fractions. After isolation (≥95% purity; ≥90% yield), the COS were examined for growth promotion of probiotic strains. Benchmarked against inulin, trans-galacto-oligosaccharides and cellobiose, COS showed up to 4.1-fold stimulation of cell density for <em>Clostridium butyricum</em>, <em>Lactococcus lactis</em> subsp.<em>lactis</em>, <em>Lactobacillus paracasei</em> subsp. <em>paracasei</em> and <em>Lb. rhamnosus</em> but were less efficient with <em>Bifidobacterium</em> sp. This study shows the COS as selectively functional carbohydrates with prebiotic potential and demonstrates their efficient enzymatic production.