Figure S1 - Englerin A Selectively Induces Necrosis in Human Renal Cancer Cells

Englerin A induced cell death follows a distinct time course and differs morphologically from staurosporine induced apoptosis. Cells were treated with either 1 μM englerin A or 5 μM staurosporine for 1 h or 3 h. After incubation, cells were trypsinized and stained for extracellular phosphatidyl serine expression using FITC-tagged Annexin V and propidium iodide (PI) as co-stain to test cell membrane integrity. Shown is a representative result of three independent experimental repeats. Dot plots show cells testing positive for Annexin V binding (early apoptotic stages) in the upper left quadrant and cells positive for Annexin V binding and propidium iodide uptake (late apoptotic stages/necrotic death) in the upper right quadrant. Numbers shown represent quadrant percentages related to total number of cells. (EPS)