Cellular population dynamics shape the route to human pluripotency. Cellular population dynamics shape the route to human pluripotency

Here we analyzed the gene expression dynamics of human cells reprogramming by using single cell RNA-seq (sc-RNAseq). Overall design: Human BJ fibroblasts were reprogrammed to induced pluripotent stem cells in microfluidics chambers through mRNA transfections. Samples were harvested before the first transfection (D0), 3 days after transfection (D3) and then every 2 days (D5, D7, D9, D11, D13 and D15). All the samples were cryopreserved and finally analyzed through single cell RNA sequencing.