ELISA of VGF peptides in mouse.

Upper panel (plasma): upon fasting (0’), higher VGF C-terminus, NAPPE, and ERVW immunoreactive peptide/s were shown in slim vs. obese mice, while the glucose load (120’) led to increased VGF C-terminus and TLQP peptide/s in slim mouse, with little response in the obese group. Middle panel (brown adipose tissue, BAT): TLQP, NAPPE and ERVW peptides were higher during fasting (0’) in slim mice, which also showed a distinct increase of VGF C-terminus peptide/s after the glucose load (120’). Lower panel (white adipose tissue, WAT): in basal conditions (0’) ERVW peptide/s levels were higher in slim mice, while the glucose load (120’) resulted in a response of all other VGF peptides in slim mice (only). VGF C-term: VGF C-terminus peptide/s; ERVW refers to the C-terminally directed assay used for QQET-30 like peptide/s (see Table 1); mean±SEM, * = P