Proteomic data were obtained from hydrogenotrophic methanogen strain isolated from the rumen of sheep under methanogenesis-inhibiting conditions, providing a resource to interrogate protein-level adjustments and methane-associated pathways during inhibitor exposure in assays.

Proteomic data were obtained from hydrogenotrophic methanogen strain isolated from the sheep rumen under methanogenesis-inhibiting conditions, providing a resource to interrogate protein-level adjustments and methane-associated pathways during inhibitor exposure. The dataset enables analyses of differential abundance and pathway enrichment linked to methane production and stress responses in controlled in vitro assays.