Whole-transcriptome profiling of laser-capture microdissected striatal neurons at single-cell resolution using retrograde tracing

Pathway-specific striatal transcripts were identified in a neuroanatomically defined manner using retrograde tracing, laser capture micodissection and whole-transcriptome sequencing. Fluorescent retrograde tracers were stereotaxically infused into striatal terminal fields and laser capture microdissection was used to isolate striatomesencephalic and striatipallidal neurons at single-cell resolution.