Targeted inhibition of kisspeptin neurons reverses hyperandrogenemia and abnormal hyperactive LH secretion in a preclinical mouse model of polycystic ovary syndrome

STUDY QUESTION Do hyperactive kisspeptin neurons contribute to abnormally high LH secretion and downstream hyperandrogenemia in polycystic ovary syndrome (PCOS)-like conditions and can inhibition of kisspeptin neurons rescue such endocrine impairments?
SUMMARY ANSWER Targeted inhibition of endogenous kisspeptin neuron activity in a mouse model of PCOS reduced the abnormally hyperactive LH pulse secretion and hyperandrogenemia to healthy control levels.
WHAT IS KNOWN ALREADY PCOS is a reproductive disorder characterized by hyperandrogenemia, anovulation, and/or polycystic ovaries, along with a hallmark feature of abnormal LH hyper-pulsatility, but the mechanisms underlying the endocrine impairments remain unclear. A chronic letrozole (LET; aromatase inhibitor) mouse model recapitulates PCOS phenotypes, including polycystic ovaries, anovulation, high testosterone, and hyperactive LH pulses. LET PCOS-like females also have increased hypothalamic kisspeptin neuronal activation which ma..., All methods and analyses are described in detail in the published manuscript Methods section., , # Targeted inhibition of kisspeptin neurons reverses hyperandrogenemia and abnormal hyperactive LH secretion in a preclinical mouse model of polycystic ovary syndrome

[https://doi.org/10.5061/dryad.pk0p2ngz7](https://doi.org/10.5061/dryad.pk0p2ngz7)

## Description of the data and file structure

The data are from a study that compared hormone levels, body and tissue weights, and mRNA levels between several groups of animals that were exposed for 5 weeks to Letrozole (LET) or placebo control and which had their kisspeptin neurons inhibited (Cre+ animals) or not (Cre- animals) by chemogenetic technology, as described in detail in the Methods section of the published manuscript.  

The dataset used to generate all graphs and figures for the associated manuscript are deposited here in an .xlsx file named  \"All_figures_data_for_LET_CNO_study.xlsx\". 

The .xlsx file comprises 13 separate worksheets named with the corresponding manuscript Figure number (e.g., Fig 2A, Fig 3, Fig 4A, etc.) whi...,