Gene expression profiling of mouse bone marrow-derived macrophages (RNA-Seq)
收藏NIAID Data Ecosystem2026-03-10 收录
下载链接:
https://www.ncbi.nlm.nih.gov/sra/SRP043011
下载链接
链接失效反馈官方服务:
资源简介:
Bone marrow (BM) cells were obtained by flushing the long bones of 8-week old C57BL/6 mice. BM cells were then were plated in macrophage SFM medium (Life Technologies) supplemented with penicillin-streptomycin and CSF-1 (Peprotech, 100 ng/ml) and cultured for one week to allow macrophage differentiation. BMDMs were polarized by adding IL-4 to the medium (40 ng/ml, Peprotech) for 72h or left untreated. Overall design: 8 samples total, 2 groups, 4 biological replicates in each group
创建时间:
2017-09-17



