Raw dSTORM Single-Molecule Localization Microscopy (SMLM) Molecule Tables for Rabbit Sinoatrial Node Cells

This dataset contains the raw Single-Molecule Localization Microscopy (SMLM) molecule tables used for analysis in the associated manuscript \"A New Fight-or-Flight Pacemaker Mechanism via Ryanodine Receptor Abundance and Super-Clustering\". The data include direct Stochastic Optical Reconstruction Microscopy (dSTORM) localizations of ryanodine receptor 2 (RyR2) channels in isolated rabbit sinoatrial node cells (SANCs). Fourteen SANCs were imaged: 6 basal cells (Cells 1–6) and 8 β-adrenergic receptor–stimulated cells (Cells 7–14), as reported in Table S2 of the manuscript. Each file corresponds to one SANC and contains the unfiltered localization coordinates and associated metadata generated by the Zeiss ZEN Black SMLM pipeline, prior to drift correction, filtering, grouping, or clustering. RyRs were labeled with the monoclonal antibody C3-33 (Thermo Fisher Scientific, MA3-916) and Alexa Fluor 647–conjugated secondary antibody, as described in the Methods. These raw SMLM molecule tables correspond to the “Raw SMLM Molecule Table” stage in the processing pipeline (Figure 1) and served as input for the subsequent filtering, DBSCAN clustering, Hopkins statistic analysis, and computational model integration described in the manuscript.