Dendritic Cell maturation/migration is architected by chromatin accessibility and a tonic organismal IFNgR1 response gradient which governs immune priming and tolerance. Dendritic Cell maturation/migration is architected by chromatin accessibility and a tonic organismal IFNgR1 response gradient which governs immune priming and tolerance

We found that Interferon gamma receptor 1 (IFNgR1) signaling regulates all aspects of tonic maturation including: tissue egress, migration, maturation, antigen capture and presentation, and subtly balances DC ontogeny. Through ATACseq analysis, we further identified that site-specific maturation is architected by chromatin accessibility and NFkB, suggesting IFNg serves as a local tissue-based maturation cue. Overall design: To study whether IFNgR1 signaling might impact organismal maturation at the level of the chromatin, we sorted different DC subsets, including resident cDC1, cDC2 and migratory migDC1, migDC2, DN migDC, out from skin draining LN of WT, IFNgR1-/-, CD11cCre-/IFNgR1flfl and CD11cCre+/IFNgR1flfl mice. ATACseq was then performed using those sorted samples to compare the chromatin accessibility landscapes between different DC subsets with or without IFNgR1 signaling.