RNA-seq of Streptococcus thermophilus N4L grown in two different yeast extracts

In the present work, we aimed at investigating how the respective composition (especially the peptide content) of two different yeast extracts influenced Streptococcus metabolism during growth. To reach that goal, we used one strain of S. thermophilus (N4L) and two yeast extract-based growth media leading to similar fermentation kinetics and yields that differed by their peptide content. Three replicate cultures were performed for each yeast extract-based growth medium, under pH control. Samples were collected after 3, 4 and 5 h of growth, cells were harvested