B6-129 MLH1 protein polymorphisms.

(A) Schematic representation of the murine MLH1 protein showing the location of B6-129 nonsynonymous polymorphisms identified (purple triangles) and their positions relative to conserved ATP binding motifs and ATP binding domain (dark and light red boxes, respectively) [46], as well as proposed MMR protein interaction domains (blue boxes) [41]. (B) Cross-species alignment of B6 and 129 MLH1 proteins in regions encompassing the polymorphic sites between the two strains. Protein sequence alignment was performed using Clustal Omega [96] and visualized in Jalview [97] with BLOSUM62 color scheme: white, residue does not match the consensus residue at that position; light blue, residue does not match the consensus residue but the two residues have a positive BLOSUM62 score; dark blue, residue matches consensus sequence.