Exploring the Mechanism of Hydroxysafflor Yellow A in Regulating Autophagy to Improve Renal Injury in Diabetic Mice

This dataset is derived from a comprehensive study combining network pharmacology and animal experiments to investigate the targets and mechanisms of hydroxysafflor yellow A (HSYA) in the treatment of diabetic kidney disease (DKD). The network pharmacology part involved obtaining potential targets of HSYA (851 targets) and DKD-related targets (4,905 targets) from public databases, yielding 402 intersecting key targets. A protein-protein interaction (PPI) network was constructed using the STRING database and Cytoscape 3.9.0, identifying core targets such as TP53, AKT1, and EP300. GO functional and KEGG pathway enrichment analyses were performed using the DAVID platform, resulting in 764 BP terms, 104 CC terms, 195 MF terms, and 177 pathways (P < 0.05). The animal experiment part employed a db/db mouse model to evaluate the protective effects of HSYA against DKD-induced renal injury. Animal experiments were conducted in an SPF-grade animal facility between 2023 and 2025.The experimental subjects were 8-week-old male db/db and db/m mice, divided into 6 groups: db/m normal control group, db/db model group, valsartan group (10.4 mg/kg), HSYA high-dose group (10 mg/kg), HSYA medium-dose group (5 mg/kg), and HSYA low-dose group (2.5 mg/kg), with 5 mice per group. All mice received continuous intragastric administration for 8 weeks. This dataset contains individual observational records from 30 mice, with each record corresponding to complete experimental data from one mouse. The row labels in the data table represent individual animal identification numbers, and the column labels include: group, body weight (g), blood glucose (mmol/L), urinary albumin (mg/L), urinary albumin-to-creatinine ratio (mg/mmol), renal tissue p-PI3K/PI3K protein expression ratio, p-AKT/AKT protein expression ratio, and p-mTOR/mTOR protein expression ratio. Western blot data were analyzed using ImageJ software for灰度值 analysis (gray value analysis), with three repeated measurements per protein sample,最终 presented as "mean ± standard deviation". All protein ratio indicators are dimensionless relative expression levels. Urinary albumin and blood glucose measurements were performed using commercial kits according to the manufacturers' instructions, with measurement units clearly标注 in the data table.This dataset is submitted as a single Microsoft Excel file (.xlsx) containing multiple worksheets: the first worksheet contains the compiled experimental data summary table; subsequent worksheets contain embedded original Western blot images (each image corresponds to the development results for one protein target). The Excel file can be opened normally with Microsoft Office 2019 or later versions, or with open-source software such as WPS Office and LibreOffice, and the embedded images can be viewed directly within the worksheets.This dataset has no systematic missing data. Data errors mainly originate from biological individual differences and operational factors such as antibody batch variation during Western blot experiments. The standard deviation range for Western blot data is generally between 5% and 15% of the mean, and the coefficient of variation for urinary albumin detection is controlled within 10%.