Ebraccatus_Priority_Effects

The EPE was a collection of short term studies in which the eggs of hourglass treefrogs were inoculated with bacteria in different orders in order to test for the presence of priority effects in tadpole bacteria community development. The first experiment was an inoculation experiment with frog embryos using two bacterial isolates. To complement our tadpole experiment, a follow-up experiment was conducted in which broth containing wells within a well-plate were similarly inoculated with bacteria under two nutrient concentrations (Full LB broth or a 1:20 dilution LB broth). We then conducted a third test in which embryos were inoculated first with a single bacterium and then with a synthetic community of bacteria.The sequences included are 16S rRNA sequences of the V4 variable region.本EPE数据集为一系列短期研究的集合，核心研究目标为通过以不同顺序将细菌接种至沙漏树蛙（hourglass treefrog）的蛙卵中，检验蝌蚪细菌群落构建过程中是否存在优先效应（priority effects）。第一项实验为采用两种细菌分离株（bacterial isolates）开展的蛙胚胎接种实验。为补充本项蝌蚪相关实验，我们开展了一项后续实验：在微孔板（well-plate）内的培养液中，以两种营养浓度（完全LB培养液（Full LB broth）或1:20稀释LB培养液（1:20 dilution LB broth））开展相似的细菌接种操作。随后我们开展了第三项测试：先向胚胎接种单一细菌，随后再接种细菌合成群落。本数据集所收录的序列为V4可变区的16S核糖体RNA（16S rRNA）序列。