ALYREF Mediates RNA m5C Modification to Promote Hepatocellular Carcinoma Progression

The RNA methyltransferase Aly/REF export factor (ALYREF) is considered one type of “reader” protein located in the nucleus that recognizes and binds directly with m5C sites in RNA and facilitates the export of RNA from the nucleus to the cytoplasm. Notably, ALYREF is considered a promising target for diagnosis and prognosis prediction. However, until now, the low number of related studies has limited the understanding of the mechanism of the HCC-promoting effects of ALYREF. To further elucidate the oncogenic roles of ALYREF in hepatocellular carcinoma (HCC), we assessed the expression levels of ALYREF in clinical samples and HCC cell lines and explored the effects of ALYREF deficiency by both in vitro experiments and m5C-methylated RNA immunoprecipitation sequencing (m5C-MeRIP-Seq) Overall design: The effect of ALYREF deficiency on Huh-7 and HepG2 cells was investigated. To further explore the potential molecular mechanism of ALYREF in RNA m5C modification. We then performed m5C-MeRIP-Seq on both sh-NC and sh-ALYREF Huh-7 and HepG2 cells. (Three samples and three controls)