Expression data from Ezh2 epithelial knock-out mouse lungs at E14.5

Ezh2 epigenetically suppresses developmentally-regulated genes. Ezh2 is highly expressed during development, including in the lung. We knocked out Ezh2 in the developing lung epithelium using a Shh-cre driver which is active in foregut endoderm prior to lung morphogenesis. Many developmentally regulated genes became derepressed in the mutant lungs, leading to defects in lung development. Microarray analysis of genes upregulated in Ezh2 epithelial knock-out mouse lungs compared to Shh-cre controls. We generated Ezh2 epithelial mutant lungs by crossing the Ezh2-floxed line (Su et al., 2003) with the Shh-cre line (Harfe et al., 2004). We isolated RNA from whole mouse lungs at embryonic day 14.5 from 4 Shh-cre:Ezh2-flox/flox embryos (M1-M4) and 4 Shh-cre embryos (C1-C4). Littermates are as follows: C1+C2; C3+C4; M1+M2; M3+M4.Ezh2（Enhancer of zeste homolog 2）可通过表观遗传调控抑制发育相关基因的表达。该基因在发育进程中广泛高表达，肺部组织亦为其高表达位点之一。我们采用在肺形态发生前即可在前肠内胚层中激活的Shh-cre（Sonic hedgehog-Cre重组酶）驱动系统，在发育中的肺上皮细胞内敲除Ezh2基因。突变肺组织中大量发育调控基因出现去抑制现象，最终引发肺发育缺陷。本数据集通过微阵列分析，对比了Ezh2上皮细胞敲除小鼠肺组织与Shh-cre对照小鼠肺组织中上调表达的基因。我们通过将Ezh2 floxed等位基因小鼠品系（Su等，2003）与Shh-cre小鼠品系（Harfe等，2004）杂交，构建了Ezh2上皮细胞突变肺组织模型。我们于胚胎发育第14.5天，从4只Shh-cre:Ezh2 flox/flox胚胎（编号M1-M4）与4只Shh-cre胚胎（编号C1-C4）的全肺组织中提取总RNA。同窝仔鼠分组如下：C1+C2；C3+C4；M1+M2；M3+M4。