Figure S1 - Regulation of Human Formyl Peptide Receptor 1 Synthesis: Role of Single Nucleotide Polymorphisms, Transcription Factors, and Inflammatory Mediators

Analysis of FPR1 expression in U937 cells by flow cytometry. U937 cells were incubated 0–5 days with 1% DMSO, as indicated in the figure. FPR1 expression was visualized using a fluorescent high affinity binding ligand, N-formyl-Nle-Leu-Phe-Nle-Tyr-Lys-fluorescein (FL-1). Propidium iodide was used to visualize the non-viable cells (FL-2). The proportion of both FPR1-positive cells and non-viable cells increased over time with maximal FPR1 expression after 4–5 day incubation.
(EPS)