Gene expression of Tg 3xFLAG-Nanos2-deltaN10 mouse male gonad

To investigate the biochemical function of NANOS2, we performed expression microarray analysis of the embryonic male gonad of Nanos2 hetero, Nanos2 KO, and Nanos2 KO_Tg 3xFLAG-tagged Nanos2-deltaN10, which is truncated form of Nanos2 in the N-terminal region, transgenic mice. 3xFLAG-tagged Nanos2-deltaN10 protein function was validated by rescue experiment in the Nanos2-null male gonad of mouse embryo at E14.5. Biological duplicates were examined at each genotype, Nanos2 hetero, Nanos2 KO, and Nanos2 KO_Tg 3xFLAG-tagged Nanos2-deltaN10 for each experiment.为探究NANOS2的生化功能，我们对三种基因型小鼠的胚胎期雄性性腺开展了基因表达微阵列分析，分别为：Nanos2杂合子（hetero）、Nanos2敲除型（KO），以及构建于Nanos2敲除遗传背景下、表达3xFLAG标签融合的N端截短型Nanos2（Nanos2-deltaN10）的转基因小鼠品系Nanos2 KO_Tg 3xFLAG-tagged Nanos2-deltaN10。其中，该3xFLAG标签融合的Nanos2-deltaN10蛋白的功能，已通过小鼠胚胎发育第14.5天（E14.5）的Nanos2缺失雄性性腺拯救实验得到验证。本实验针对每一种基因型均设置了两次生物学重复，每个实验组均完成了重复检测。