Confirmation of MOR-MORIP interactions.

1MORIPs isolated either in traditional or MYTH screens were tested for interaction with each of the intracellular loops (IL1, 2, or 3) or carboxyl-terminus (C-tail) of the MOR in a directed Y2H assay.2GST PD: Full-length MORIPs were tested for interaction with the MOR-IL2 in a GST pulldown assay.3Co-IP: Full-length MORIPs were tested for association with the delta (DOR), kappa (KOR), or mu (MOR) opioid receptor by co-immunoprecipitation.+indicates a positive result, - indicates a negative result, while ND indicates not done.