Interaction of DEPDC7 with CARMA2sh in the yeast two-hybrid assay.

Yeast AH109 was transformed with DEPDC7 fused to the GAL4-TAD, together with the indicated cDNAs fused to GAL4-DBD. The cDNA encoding for FADD served as a negative control. The interactions were examined by yeast growth on selective media; assays were done for five to ten independent transformants. Yeast colonies were scored as positive when growth developed within 24–36 hrs; a negative was scored when growth failed to develop within 1 week.Interaction of DEPDC7 with CARMA2sh in the yeast two-hybrid assay.