Comparative transcriptional signatures provide uncovering major molecular processes in early and mature somatic cyst cells of Drosophila testes. Comparative transcriptional signatures provide uncovering major molecular processes in early and mature somatic cyst cells of Drosophila testes

Here we employed a FACS-based approach for isolation of early and mature somatic cyst cells from whole testes of adult Drosophila males. We prepared and analyzed RNA-seq libraries to reveal differences in transcriptional patterns of these cells and to uncover protein complexes functioning in cyst cells at different stages of spermatogenesis. Overall design: Purified PolyA(+) mRNA libraries from early and mature somatic cyst, and from control unsorted cells of Drosophila testes