Comparison of murine colonic mucosal gene expression between postanatal day 90 (P90) to postnatal day 30 (P30)

Comparison of murine colonic mucosal gene expression between postnatal day 90 (P90) to postnatal day 30 (P30) by whole genomic expression microarray. Gene expression profiling of colonic mucosal DNA between P90 and P30 mice. Agilent Technologies two-color labelling kit and genomic hybridization protocol was utilized. 4 independent P90 to P30 comparisons were performed as follows. RNA (0.4ug) samples were processed and labelled with Cy3 and Cy5 (Quick Amp labelling kit, two color, Agilent technologies) and 4 independent P90 to P30 comparisons were done on Agilent tecnologies 4x44k whole genomic expression microarray G2519F, Amadid:014868)本研究通过全基因组表达微阵列（whole genomic expression microarray）技术，对比出生后第90天（P90）与出生后第30天（P30）小鼠的结肠黏膜基因表达差异。同时对P90与P30小鼠的结肠黏膜脱氧核糖核酸（DNA）开展基因表达谱分析。本研究采用安捷伦科技（Agilent Technologies）双色标记试剂盒及基因组杂交实验方案完成实验操作。共设置4组独立的P90与P30对照实验，具体流程如下：取0.4μg的核糖核酸（RNA）样本进行处理并以Cy3与Cy5荧光标记，标记试剂采用安捷伦科技Quick Amp双色标记试剂盒；随后在安捷伦科技4x44k全基因组表达微阵列G2519F（Amadid编号：014868）上完成4组独立的P90与P30对照杂交实验。