ER-Apex2 Mass Spectrometry Protein Identification Dataset

HeLa cells were transfected with ER-APEX2. The next day, the labeling protocol detailed in Hung, Victoria et al. Nature protocols vol. 11,3 (2016): 456-75. by incubating the cells with 500 uM biotin-phenol for 30 minutes before being treated with a final concentration of 1 mM hydrogen peroxide. A quenching buffer was used to stop the reaction after 1 minute. The cells were then lysed in BioID buffer and the lysate was used for an immunoprecipitation with streptavidin beads. An SDS page gel was run with the resulting elution sample. The gel was stained with Coomassie blue. The appropriate lane was excised and submitted for mass spectrometry analysis. The mass spectrometry analysis was performed by the Mass Spectrometry (MS) & Proteomics Resource of the W.M. Keck Foundation Biotechnology Resource Laboratory located at the Yale School of Medicine. Details of the mass spectrometry analysis parameters are in the excel file "ER-Apex2 MassSpectrometryData."