Thermal performance of Aedes sierrensis life history traits for populations collected across the species range

How mosquitoes may respond to rapid climate warming remains unknown for most species, but will have major consequences for their future distributions, with cascading impacts on human well-being, biodiversity, and ecosystem function. We investigated the adaptive potential of a wide-ranging mosquito species, Aedes sierrensis, across a large climatic gradient by conducting a common garden experiment measuring the thermal limits of mosquito life history traits. Although field-collected populations originated from vastly different thermal environments that spanned over 1,200 km, we found limited variation in upper thermal tolerance between populations. In particular, the upper thermal limits of all life history traits varied by <3°C across the species range and, for most traits, did not differ significantly between populations. For one life history trait—pupal development rate—we did detect significant variation in upper thermal limits between populations, and this variation was strongly ..., This dataset pertains to life history trait measurements made on 10 populations of Aedes sierrensis collected from across western North America. Mosquitoes were held at six tempreature treatments, and life history traits (larval and pupal survival and development rates, and adult lifespan) were made through tracking individuals daily. This data was used to obtain estimates of thermal limits for each population and trait using Bayesian analysis. Further details of mosquito collection, rearing, data collection, processing, and analysis are available in the manuscript, as well as a preprint: https://www.biorxiv.org/content/10.1101/2023.03.02.530886v2, , # Thermal performance of *Aedes sierrensis* life history traits for populations collected across the species range

These are the scripts and data used for analysis in the manuscript '**Mosquito thermal tolerance is remarkably constrained across a large climatic range'** by Lisa Couper, Johannah Farner, Kelsey Lyberger, Alexandra Lee, and Erin Mordecai. The manuscript investigates the evidence for mosquito thermal adaptation using *Aedes sierrensis* as a model system. We collected 10 populations of Aedes sierrensis from tree holes across western North America (spanning 1,200 km), then reared all individuals in the lab for one generation under common temperatures. We then separated F1 individuals into one of six temperature treatments ranging from 5-32℃ and tracked individuals daily to measure individual life history traits including larval and pupal survival and development rates, and adult lifespan. We then fit thermal performance curves to these experimental data to estimate upper a...