Thermal performance of Aedes sierrensis life history traits for populations collected across the species range

How mosquitoes may respond to rapid climate warming remains unknown for most species, but will have major consequences for their future distributions, with cascading impacts on human well-being, biodiversity, and ecosystem function. We investigated the adaptive potential of a wide-ranging mosquito species, Aedes sierrensis, across a large climatic gradient by conducting a common garden experiment measuring the thermal limits of mosquito life history traits. Although field-collected populations originated from vastly different thermal environments that spanned over 1,200 km, we found limited variation in upper thermal tolerance between populations. In particular, the upper thermal limits of all life history traits varied by <3°C across the species range and, for most traits, did not differ significantly between populations. For one life history trait—pupal development rate—we did detect significant variation in upper thermal limits between populations, and this variation was strongly ..., This dataset pertains to life history trait measurements made on 10 populations of Aedes sierrensis collected from across western North America. Mosquitoes were held at six tempreature treatments, and life history traits (larval and pupal survival and development rates, and adult lifespan) were made through tracking individuals daily. This data was used to obtain estimates of thermal limits for each population and trait using Bayesian analysis. Further details of mosquito collection, rearing, data collection, processing, and analysis are available in the manuscript, as well as a preprint: https://www.biorxiv.org/content/10.1101/2023.03.02.530886v2, , # Thermal performance of *Aedes sierrensis* life history traits for populations collected across the species range These are the scripts and data used for analysis in the manuscript '**Mosquito thermal tolerance is remarkably constrained across a large climatic range'** by Lisa Couper, Johannah Farner, Kelsey Lyberger, Alexandra Lee, and Erin Mordecai. The manuscript investigates the evidence for mosquito thermal adaptation using *Aedes sierrensis* as a model system. We collected 10 populations of Aedes sierrensis from tree holes across western North America (spanning 1,200 km), then reared all individuals in the lab for one generation under common temperatures. We then separated F1 individuals into one of six temperature treatments ranging from 5-32℃ and tracked individuals daily to measure individual life history traits including larval and pupal survival and development rates, and adult lifespan. We then fit thermal performance curves to these experimental data to estimate upper a...多数蚊类物种对快速气候变暖的响应机制仍未明确，但该响应将对其未来分布范围产生重大影响，并进而对人类福祉、生物多样性及生态系统功能产生级联效应。本研究以分布广泛的塞氏伊蚊（Aedes sierrensis）为研究对象，通过开展共同花园实验（common garden experiment）测定蚊类生活史性状的热极限，探究其在大型气候梯度下的适应潜力。尽管野外采集的种群来自跨度超过1200公里、热环境差异极大的区域，但我们发现不同种群间的上限热耐受能力仅存在有限差异。具体而言，在该物种的分布范围内，所有生活史性状的上限热极限差异均小于3℃，且多数性状在种群间无显著差异。针对蛹发育速率这一生活史性状，我们确实检测到不同种群间的上限热极限存在显著差异，且该差异呈现显著……（原文未完成） 本数据集涉及从北美西部全域采集的10个塞氏伊蚊种群的生活史性状测定数据。研究人员将蚊幼虫置于6种温度处理条件下饲养，并通过每日追踪个体，测定其生活史性状（包括幼虫与蛹的存活率、发育速率以及成虫寿命）。本数据集通过贝叶斯分析（Bayesian analysis）为每个种群及性状估算了热极限参数。有关蚊类采集、饲养、数据收集、处理与分析的更多细节，可参见已发表论文及预印本：https://www.biorxiv.org/content/10.1101/2023.03.02.530886v2。 # 跨分布范围采集的塞氏伊蚊种群生活史性状的热性能 本数据集包含Lisa Couper、Johannah Farner、Kelsey Lyberger、Alexandra Lee与Erin Mordecai发表的论文《蚊类在大范围气候梯度下的热耐受能力显著受限》（*Mosquito thermal tolerance is remarkably constrained across a large climatic range*）中分析所用的代码与数据。该论文以塞氏伊蚊为模式系统，探究蚊类热适应的相关证据。研究团队从北美西部跨度1200公里的树洞中采集了10个塞氏伊蚊种群，并在实验室统一温度条件下将所有个体饲养至F1代。随后将F1个体分为6组温度处理（温度范围为5℃至32℃），并每日追踪个体以测定其生活史性状，包括幼虫与蛹的存活率、发育速率以及成虫寿命。随后我们针对这些实验数据拟合热性能曲线（thermal performance curves），以估算上限……（原文未完成）