Single-cell RNA sequencing of the rat carotid arteries uncovers mechanisms of in-stent restenosis

Maps of heterogeneous cellular landscape in carotid artery were defined by single-cell RNA sequencing and revealed several cell types with their internal relations in the ISR model. This study highlights the crucial role of VSMC phenotype switching in the progression of ISR, and provides the clues of the underlying mechanism of ISR.本研究通过单细胞RNA测序（single-cell RNA sequencing）绘制了颈动脉异质性细胞图谱，并揭示了支架内再狭窄（In-stent Restenosis, ISR）模型中多种细胞类型及其内在关联。本研究凸显了血管平滑肌细胞（vascular smooth muscle cell, VSMC）表型转化在ISR进展中的关键作用，同时为ISR的潜在发病机制提供了研究线索。